Metallothionein-1 isoforms and vimentin are direct pu.1 downstream target genes in leukemia cells*

HIGHLIGHTS

• who: Akemi Imoto from the chostatin A (TSA, Sigma) at, , .5, or , ␮M. The cells were harvested after , h for, azadC treatment and , h for SB and TSA treatments. cDNAs were prepared from the cells using reverse transcriptase (Superscript II, Invitrogen). Quantitative PCR was performed using the Quantitect SYBR Green PCR Reagent (Qiagen) according to the manufacturer's protocol and an Opticon Mini Real-time PCR Instrument (Bio-Rad, Hercules, CA) as previously described (19, ). The sequences of the primers were: MT- G: forward (5⬘-CTTCTCGCTTGGGAACTCTA-3⬘) and reverse (5⬘-AGGGGTCAAGATTGTAGCAAA-3⬘) (21) have published the article . . .
  

   

  

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