Method combining bac film and positive staining for the characterization of dna intermediates by dark-field electron microscopy

HIGHLIGHTS

  • who: Biology Methods et al. from the Université Paris-Saclay, Roussy, Villejuif , France and UMR9019-CNRS have published the research: Method combining BAC film and positive staining for the characterization of DNA intermediates by dark-field electron microscopy, in the Journal: (JOURNAL) of 19/06/2020
  • what: This work describes the technical optimizations the authors have developed using a rapid and reproducible sample preparation procedure to characterize dsDNA as well as ssDNA, mixed ss-dsDNA and branched DNA from synthetic DNA substrates to in_vivo DNA structures formed along DNA transactions.

SUMMARY

     

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