HIGHLIGHTS
- who: Elio Berutti from the Mandibular first molars with a fully formed apex that had not previously undergone endodontic treatment, and that had been freshly extracted for periodontal reasons, were selected in accordance with the local ethics committeeThe patients provided an informed consent for their extracted teeth to be used for research purposes. The teeth were free of caries, cracks, and artificial alterations. A sample size of , was calculated with G*Power, .1., (Kiel University, Kiel, Germany) ANOVA test to set the study power at, % considering an alpha-error=0.05. After debridement of the root surface . . .
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