Microrna-21 is immunosuppressive and pro-metastatic via separate mechanisms

HIGHLIGHTS

• who: Lap Hing Chi from the Generation of stably transduced cell lines, cl4-mCherry and EO LMB-mCherry cells were transduced with pMSCVpuro-mi, (a gift from Andrei Goga (University of California, San Francisco) or empty vector retrovirus and selected for stable integration using puromycin (10μg/ml). Stably transduced cells were maintained in puromycin (5μg/ml). Knockdown of mi, function in cells was achieved using the miArrestTM lentiviral vector system (pEZX-, vector backbone, GeneCopoeia, Rockville, MD, USA) that features the mCherry fluorescent reporter, the hygromycin B resistance gene, and a mature mi, sequestering RNA sequence . . .
  

   

  

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