Microrna-375 is a therapeutic target for castration-resistant prostate cancer through the ptpn4/stat3 axis

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• who: Junqing Gan from the Isolation and identification of hucMSCs Fresh human umbilical cords were obtained from the Second Affiliated Hospital of Harbin Medical University with informed consentMesenchymal progenitor cells were cultured in serum-free MesenCult-ACF Plus Medium (05448, STEMCELL, Canada) immediately after the umbilical cord had been harvested. After perivascular Wharton's jelly was incubated in, % , at , °C for, days until the cells (P0) were confluent around the colonization point, the hucMSCs were passaged, and the hucMSCs at the, th passage were used for further analysis and experiments. Flow cytometry was used to detect the . . .
  

   

  

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