Microtubule motors regulate isoc activation necessary to increase endothelial cell permeability

HIGHLIGHTS

• who: Songwei Wu from the To image endoplasmic reticulum distributions in cultured cells, ER Tracker (Molecular Probes, Eugene, OR) was added to culture medium at , ␮mol/liter for , min, and then the cells were observed by epifluorescence microscopy without fixationImmunoblot Analysis-Immunoblotting using antibody against ␣-tubulin was used to measure tubulin monomer and polymer levels in cultured PAECs and PMVECs. These studies were performed essentially as outlined previously by Minotti et al. (26) and Marklund et_al (27). Briefly, the cells were plated and cultured as detailed above, and then thapsigargin, rolipram, or both compounds were added. The . . .
  

   

  

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