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- who: Antonella De Cola from the BCSCs were isolated collecting human breast cancer tissues at the Department of Surgical, Oncological and Stomatological Sciences (University of Palermo, Palermo, Italy), in accordance with the ethical standards of the Institutional Committee on Human Experimentation and their molecular subtypes Breast cancer tissues were digested as previously described6, and cells were cultured in ultra-low attachment flasks in a selective medium (serum-free) supplemented with , ng/mL fibroblast growth factor (βFGF) (Peprotech), ng/mL epidermal growth factor (EGF) (Peprotech) at , °C in a, % (v/v) , humidified chamber. In this culture condition, breast . . .
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