Mir-455-5p suppresses

HIGHLIGHTS

• who: Sheng-Yen Hsiao from the The pLe-miR-455-5p plasmids were donated by DrShine-Gwo Shiah [13]. Short hairpin (sh) RNAs against PDZK , were constructed. The target sequences of shPDZK IP1, and sh-PDZK IP1, were, u2032-TTCu200bCTGu200b GTCu200bCTCu200bGTTu200bGCAu200bATC-3u2032 and, u2032- TTGu200bGAGu200bCTC-AGAu200b CCGu200bTGTu200bCTAu200b-3u2032, respectively. PLAS2w.Pneo vectors obtained from the National RNAi Core Facility (Academia Sinica, Taiwan) were used to induce , overexpression. Lentiviruses were manipulated in accordance with standard protocols (RNAi Core Laboratory of the Research Center of Clinical Medicine, National Cheng Kung University Hospital, Tainan, Taiwan). Luciferase reporter assay have published . . .
  

   

  

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