Mir-659-3p inhibits osteosarcoma progression and metastasis by inhibiting cell proliferation and invasion via targeting

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  • who: Yubao Gong from the All animal studies were approved by the Jilin University Institutional Animal Care and Use CommitteeSix-weekold female Balb/c athymic nude mice were purchased from Shanghai Experimental Animal Center of Chinese Academy of Sciences. During the experiments, the mice were maintained in a special pathogen-free (SPF) grade facility. HOS osteosarcoma cells were cultured in , mm cell culture dishes until about, % confluent and were collected from culture dishes with, .05% Trypsin (Life technology). The cells were re-suspended in serum-free medium at , ­ , cell/ml final concentration. Nude mice were randomly separated . . .

     

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