Mms22l-tonsl functions in sister chromatid cohesion in a pathway parallel to dscc1-rfc

HIGHLIGHTS

• who: from the The RPE1-iCas9-DSCC1-KO CRIPSR screen was performed in three independent replicates at an average, fold sgRNA representationBriefly, three DSCC1-KO cell populations were transduced in parallel in medium containing , u00b5g/ml polybrene with the lentiviral TKOv, library (a kind gift from K Chan, A Tong, and J Moffat [Donnelly Centre, University of Toronto]) at a multiplicity of infection of ~0.2. After selection with , u00b5g/ml puromycin, t=, samples were taken and Cas, was induced by addition of , ng/ml doxycycline. Cells were allowed to proliferate for ~, population doublings for about , wk . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or E-mail:

  If you don't have an account, you can create one here.