Modular reconstruction and optimization of the trans-4-hydroxy-l-proline synthesis pathway in escherichia coli

HIGHLIGHTS

  • who: Zhenyu Zhang from the Laboratory stock have published the research work: Modular reconstruction and optimization of the trans-4-hydroxy-L-proline synthesis pathway in Escherichia coli, in the Journal: (JOURNAL)
  • what: The authors aimed to improve the availability and balance of precursors to satisfy t4Hyp manufacturing requirements. At the same time, the authors focused on the hydroxylation capacity of the engineered strain; that is, the authors optimized P4H expression. To improve u03b1-KG accumulation, the authors focused mainly on the central metabolic pathways by altering u03b1-KG and isocitrate metabolism to increase the . . .

     

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