Molecular and immunological characterization of the first allergenic lipocalin in hamster

HIGHLIGHTS

• who: José Alberto Torres from the ELISA-For ELISAs, well flat bottom plates were used (Immulon HBX, Thermo Scientific)The plates were coated in duplicate overnight at , °C with , ␮g SH extract/well or, ., ␮g of Pho s, kD/well. ELISAs were carried out as described previously (11). Protein Identification and Characterization by Mass Spectrometry-The proteins were identified by MS and/or MS/MS as described previously (10). This procedure was performed in the Proteomics Facility of the Universidad Complutense de Madrid (a member of the ProteoRed-ISCIII Network). BasoTest Assays-A basophil activation test was . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.