Molecular architecture and gating mechanisms of the drosophila trpa1 channel

HIGHLIGHTS

• who: Xiaofei Wang from the The original coding sequence for the Drosophila fulllength dTRPA1-A isoform was a gift from the William Daniel Tracey lab in Indiana University and was subcloned into pEG BacMam vector with an, terminal FLAG-tag The baculovirus was generated using Sf, cells (Invitrogen) and then applied to HEK F cells (Sino Biological Inc.) in suspension for protein expression, cultured in SMM, TI medium (Sino Biological Inc.) in a cell shaker (Shanghai Zhichu Instruments) operating at , rpm, u00b0C and supplemented with, % CO2, when the density reached , u00d7 , cells/mL. After a , h shaking . . .
  

   

  

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