Molecular basis for polysaccharide recognition and modulated atp hydrolysis by the o antigen abc transporter

HIGHLIGHTS

• who: Nicholas Spellmon from the Lipopolysaccharide and O polysaccharide isolation and purification Aquifex aeolicus biomass was purchased from Harald Huber (University of Regensburg, Germany)Cells were extensively washed in PBS buffer. LPS was extracted using the hot phenol/water method (Westphal and Jann, ) supplied with, % sodium, lauroylsacosine. The water phase was collected, and the extraction was carried out two more times with the addition of, %, lauroylsarcosine sodium salt in water. The water phases were dialyzed (14, MWCO), freeze-dried, and washed with, :, (v/v) ethanol in water at , u00b0C. Nucleic acids and proteins were removed by , h . . .
  

   

  

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