Molecular basis for the interaction of [nle4,d-phe7]melanocyte stimulating hormone with the human melanocortin-1 receptor (melanocyte α-msh receptor)*

HIGHLIGHTS

• who: Ying-kui Yang from the sequencing using Sequenase Version , (Life Science Products, Cleveland, OH). The mutant receptors were then excised from , using the restriction enzymes EcoRI and SalI and subcloned into the eukaryotic expression vector pBK-CMV (Stratagene, La Jolla, CA). Large scale plasmid preparations were performed after amplifying the receptor containing plasmids in Escherichia coli strain XL1-Blue (Stratagene) using an alkaline lysis method followed by purification of closed circular DNA over a CsCl gradient (15). Cell Transfection and Culture-For these studies HEK , cells were transfected with receptor plasmid DNA using , ml of LipofectAmine . . .
  

   

  

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