Molecular basis of scrapie strain glycoform variation*

HIGHLIGHTS

• who: Ina Vorberg from the Preparation of Brain Homogenates-The mouse-adapted scrapie strains ME7, V, and L were the kind gift of DrJames Hope (Vitechnologies, Boston, MA) and were passaged once in C BL/, (ME7, L) or VmDk mice ( V). The mouse-adapted scrapie isolate RML Chandler (RML) was passaged in RML mice. Brains were stored at ⫺, °C, and brain homogenates (10% w/v) were prepared fresh in phosphate-buffered saline (PBS) containing complete protease inhibitors (Roche Molecular Biochemicals) according to a previously published protocol (29). Preparation of Cell Lysates-The mouse neuroblastoma cell line Mo . . .
  

   

  

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