Molecular characterization of a family 5 glycoside hydrolase suggests an induced-fit enzymatic mechanism

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  • who: Marcelo V. Liberato from the São Carlos InstituteUniversity of have published the Article: Molecular characterization of a family 5 glycoside hydrolase suggests an induced-fit enzymatic mechanism, in the Journal: Scientific Reports Scientific Reports
  • what: SAXS experiments were conducted to assess BlCel5B conformational states in solution, and the results revealed the enzyme in its monomeric form, with average values of Rg=27.17 Å and Dmax=87.59 Å (Supplementary Table 2). Based on the results of the crystallographic , computer simulation, and SAXS structural analyses, as well as site-directed_mutagenesis and activity assays, the authors . . .

     

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