Molecular study of the presence and transcriptional activity of hpv in semen

HIGHLIGHTS

SUMMARY

    In fact, quantity of HPV-DNA could be underestimated due to various factors, such as high sample DNA that may interfere with primer and probe binding to target DNA, poor quality of sample DNA, presence of inhibitors of PCR in anogenital secretions which reduce efficiency of amplification and/ or HPV polymorphisms that reduce binding of primers or probes to target sequences impairing amplification. As this may have been influenced by the relatively low percentage of patients with HPV-positive semen samples, the authors cannot rule out whether inflammatory action of HPV infection in the . . .

     

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