HIGHLIGHTS
- who: Dustin C. Becht from the ChIP-qPCR and ChIP-seq The eluted material obtained by fanChIP was extracted by phenol/ chloroform/isoamyl alcoholDNA was precipitated with glycogen (Nacalai Tesque), dissolved in TE, and analyzed by qPCR and deep sequencing. For deep sequencing, Purified DNA was further fragmented (~, bp long) using the Covaris , DNA shearing system (MandM Instruments Inc.). Deep sequencing was performed using a TruSeq ChIP Sample Prep Kit (illumina) and HiSeq, (illumina) at the core facility of Hiroshima University and the University of Tokyo. Data were visualized using the Integrative Genome Viewer (Broad Institute). Raw . . .
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