HIGHLIGHTS
SUMMARY
(Tracheal Volume=Segment Volume) x 100=Tracheal Volume Percent Spiracle imaging Using light and confocal microscopy, the authors imaged the spiracles on segments III and VI in the larva, pupa, adult male, and adult female, and segment I in the adult male of P. pyralis to visualize the structure of the spiracular opening and assess whether differences were present across life stages. The volume of the tracheal system in luminous segments relative to the size of the segment is considerably higher in the adult male, but this same level of increase is not seen . . .
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