Multiple cis-elements mediate the transcriptional activation of human fra-1 by 12-o-tetradecanoylphorbol-13-acetate in bronchial epithelial cells*

HIGHLIGHTS

• who: Pavan Adiseshaiah from the fraction of the soluble chromatin (1%) was saved for total chromatin inputChromatin was precleared with salmon sperm DNA/protein Aagarose for , min and then incubated with specific antibodies for , h at , °C. The immunoprecipitated products were washed, and DNA was eluted. DNA from the immunoprecipitated products was extracted after proteinase K digestion and used for PCR (, cycles) with fra, promoter-specific primers: ⫺, F (5⬘-GCGGAGCTCGCAGAAACGGAGG3⬘) and ⫺, R (5⬘-GGCGCTAGCCCCCTGACGTAGCTGCCCAT-3⬘). These primers generate a, bp DNA product. Plasmids and Mutagenesis-The, ⬘-flanking region of human fra, (⫺, to ⫹, bp) was amplified with Pfu . . .
  

   

  

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