Multiple s-layer proteins of brevibacillus laterosporus as virulence factors against insects

HIGHLIGHTS

• who: Luca Ruiu from the in ice-cold , M NaCl. Each suspension was whirl-mixed-sonicated and centrifuged at, for , min. The pellet was resuspended in, % SDS, .01% u03b2-mercaptoethanol (Merck, Darmstadt, Germany), before being boiled for , min and re-centrifuged at, for , min. The resulting supernatant was collected for analysis. One-dimensional gel electrophoresis (1-DE) protein separation analyses were conducted by mixing various protein samples with Laemmli buffer (Merck, Darmstadt, Germany) [32], boiling for , min, and running in a, % SDS-PAGE gel employing a MiniProtean electrophoresis system (BioRad Laboratories Inc., USA). Gels were digitized after . . .
  

   

  

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