Munc-18-1 inhibits phospholipase d activity by direct interaction in an epidermal growth factor-reversible manner*

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• who: Hye Young Lee from the Identification of Protein by Peptide Mass Fingerprinting Using Matrix-assisted Laser Desorption Ionization (MALDI) Time-of-flight Mass Spectrometry-After silver staining, candidate bands were excised from the gel and digested with trypsinA, ␮l aliquot of the total , ␮l of digest was used for peptide mass fingerprinting. Masses of the tryptic peptides were measured using a Bruker Reflex III mass spectrometer, as previously described (17, ). MALDI was performed using ␣-cyano4-hydroxycinnamic acid as the matrix. Trypsin autolysis products were used for internal calibration. Delayed ion extraction resulted in peptide masses with . . .
  

   

  

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