Murine frizzled-1 behaves as an antagonist of the canonical wnt/β-catenin signaling*

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• who: Sergio Roman-Roman from the for firefly luciferase and then for Renilla luciferase activityFirefly luciferase activity was normalized to Renilla luciferase activity. Plasmids and Constructs-mFz, and human Frizzled, (hFz1) were amplified by RT-PCR, and the nucleotide sequence was confirmed by DNA sequence analysis and subcloned with a Myc tag into pcDNA3., vector and into pTracer to co-express GFP protein (Invitrogen). Murine TCF expressing vector and the TCF-luciferase reporter construct, TOPflash (26), were obtained from Upstate Biotechnology. Human ␤-catenin was RT-PCR amplified, confirmed by sequencing, and subcloned into pcDNA3.1. QuikChange was . . .
  

   

  

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