Myosin x regulates sealing zone patterning in osteoclasts through linkage of podosomes and microtubules*

HIGHLIGHTS

• who: Brooke K. McMichael from the EXPERIMENTAL PROCEDURES Reagents-Affinity-purified chicken antibody against mouse Myo, was previously described (26)The specificity of this antibody in immunocytochemistry is demonstrated in supplemental Fig., showing lack of reactivity by preimmune serum as well as loss of specific labeling in Myo, siRNAtreated cells. In addition, supplemental Fig. , shows a Western blot of lysate from mature osteoclasts probed with the affinitypurified antibody. This Western demonstrates that murine osteoclasts express full-length Myo, (at ⬃, kDa), consistent with results seen in macrophages (16). Minor bands represent breakdown products that are routinely seen in . . .
  

   

  

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