Mypt1-pp1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis

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• who: Hiroki Takahashi from the Immunoprecipitation and immunoblotting analyses Whole cell lysates (WCLs) were collected in cell lysis buffer A (, mM Tris-HCl, pH, ., mM ethylenediaminetetraacetic acid (EDTA), % glycerol, mM KCl, .1% NP-40) containing protease inhibitor cocktail (Nacalai Tesque), mM phenylmethylsulfonyl fluoride, and phosphatase inhibitors (, mM NaF and , mM Na VO4). JMJD A protein was immunoprecipitated in cell lysis buffer A for , h at , u00b0C using the antibodies described in the legend to figures. Co-immunoprecipitates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the immunoblots were visualized by chemiluminescence using Super . . .
  

   

  

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