N- and c-terminal transactivation domains of gata1 protein coordinate hematopoietic program*

HIGHLIGHTS

• who: Hiroshi Kaneko from the measured using Dual-Luciferase reporter assay system (Promega) with luminometer (Berthold)pEGFP-, (Clontech) was occasionally co-transfected to measure the level of protein expression. Immunoblot analysis was performed using anti, (Santa Cruz Biotechnology) and anti-GFP (Medical and Biological Laboratories Co. Ltd.) antibodies. Recombinant Proteins, DNA Binding Assays, and Self-association Assays-Maltose-binding protein (MBP)-fused , and GST-fused , were produced in Escherichia coli BL21(DE3)pLysS-competent cells (Novagen) as described (19) and purified using glutathione-Sepharose beads (GE Healthcare) and amylose resin (New England Biolabs). Protein concentration was determined . . .
  

   

  

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