Navigating diversity: primer impact on arthropod recovery in molecular environmental monitoring in tropical amazon

HIGHLIGHTS

• What: Methods: Here, the authors assess the performance of four metabarcoding primer sets, two COI markers (ZBJ-ArtF1c/ZBJ-ArtR2c : 157bp, targeting arthropods, and mlCOIintF/jgHCO2198 : 313bp, targeting metazoans) and two 16S markers (Ins16S_1shortF/Ins16S_1shortR : 150bp, targeting insects, and Coleop_16Sc/Coleop_16Sd : 105bp, targeting arthropods, mainly Coleoptera) in amplifying the taxonomic constituents of bulk arthropod samples, collected across different natural and anthropogenic habitats from the Brazilian Amazon biome. The authors aim to tackle the intricacies of DNA metabarcoding by comparing the performance of two distinct regions, COI and 16S, in surveying the diversity of arthropods in environmental monitoring . . .
  

   

  

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