Necessity of integrated genomic analysis to establish a designed knock-in mouse from crispr-cas9-induced mutants

HIGHLIGHTS

• who: Masahide Yoshida from the DivisionMedical University have published the research work: Necessity of integrated genomic analysis to establish a designed knock-in mouse from CRISPR-Cas9-induced mutants, in the Journal: Scientific Reports Scientific Reports
• what: In this study PCR products from the 5` and 3` regions were detected using the external and internal primer pairs even in the two-copy mutant, suggesting that conventional PCR alone did not guarantee a designed one-copy mutant. In the present study, lssDNA was used as the donor DNA, and no unexpected mutations were found in any . . .
  

   

  

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