Neuroprotective actions of a fatty acid nitroalkene in parkinson’s disease

HIGHLIGHTS

• who: Roberto Maio from the Cell cultures, A rat dopaminergic neural cells (provided by DrCurt FreedUniversity of Colorado), were cultured in poly-D-lysine hydrobromide coated well plates with RPMI , medium with, glutamine, % fetal bovine serum (FBS), U/mL Pen-Strep and maintained at , u00b0C, and, % CO2. When cells reached confluency, the medium was replaced with RPMI, % FBS to minimize FBS-related scavenging of, NO2-OA and treatments were performed over , h. Then, cells were harvested or fixed to perform the assays. Cell viability Viability of, A cells was evaluated by MTT assay. Briefly, cells were seeded . . .
  

   

  

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