New, sensitive fluorogenic substrates for human cathepsin g based on the sequence of serpin-reactive site loops*

HIGHLIGHTS

• who: Sophie Re̕hault from the Materials, Human cathepsin G (EC, .21.20) was from ICN Pharmaceuticals, bovine chymotrypsin (EC, .4.21.1) was from Roche Molecular Biochemicals, and human recombinant chymase (EC, .4.21.39) was kindly provided by Prof. Norman Schechter (Dept. of Dermatology, University of Pennsylvania, Philadelphia, PA). h, was purified as described previously (23). N, Dimethylformamide and acetonitrile were from Merck, and , cartridges for reverse-phase chromatography were from Brownlee Laboratories. All other reagents were of analytical grade. Design and Synthesis of Quenched Fluorescent Substrates-All quenched fluorogenic substrates were synthesized by solid . . .
  

   

  

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