New-to-nature co2-dependent acetyl-coa assimilation enabled by an engineered b12-dependent acyl-coa mutase

HIGHLIGHTS

• What: To overcome this loss of carbon, the authors develop a new-to-nature pathway, the Lcm module, in this study. The authors demonstrate Lcm activity of the scaffold enzyme 2hydroxyisobutyryl-CoA mutase from Bacillus massiliosenegalensis, and further improve catalytic efficiency 10-fold by combining in_vivo targeted hypermutation and adaptive evolution in an engineered Escherichia coli selection strain. In a proof-of-principle, the authors demonstrate the complete Lcm module in_vitro. The work demonstrates a synthetic CO2-incorporating acetylCoA assimilation route that expands the metabolic solution space of central carbon metabolism, providing options for synthetic biology and . . .
  

   

  

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