HIGHLIGHTS
- who: Giuseppa Grasso from the (ThermoScientific, ) for , h at, u00b0C on a rotating wheelWashes were carried out , times as aforementioned and elutions were performed using HA elution buffer (, ng/ u03bcl HA peptide (ThermoScientific, ), diluted in IP buffer) for , h at, u00b0C on a rotating wheel. Following elution, beads were removed using Pierceu2122 Centrifuge Columns (ThermoScientific, ), as specified by manufacturer`s instructions. Silver-staining was performed according to the manufacturer`s instructions (Silverquest, Invitrogen). Mass spectrometry was performed at Taplin facility, Harvard University, Boston, MA. Glycerol gradient sedimentation have published the Article: NF90 interacts with components of RISC . . .
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