Nicotinic receptor subunit α5 modifies assembly, up-regulation, and response to pro-inflammatory cytokines*

HIGHLIGHTS

• who: Lorise C. Gahring from the EXPERIMENTAL PROCEDURES Materials-All of the reagents were identical to those reported previously (19)In all cases nAChR subunit-specific antibodies were prepared for epitopes in the cytoplasmic domain, and their use has been described in detail (4, , , ). In this study rabbit polyclonal , or , (␣4), or , (␤2), (␤4), and , (␣5) were used. The monoclonal antibody to the, b epitope (, ) and construction of epitope-tagged ␣, (termed ␣, b) were also described (13). Secondary antibodies were from Jackson ImmunoResearch (West Grove, PA). Expression Vectors-In all cases rat nAChR subunit cDNAs within the pcDNA3 . . .
  

   

  

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