Nodal signaling regulates asymmetric cellular behaviors, driving clockwise rotation of the heart tube in zebrafish

HIGHLIGHTS

• who: Hinako Kidokoro from the ZebrafishZebrafish (Danio rerio) were maintained under standard condition. All experiments using zebrafish were approved by the Animal Experimentation Committee of the National Cerebral and Cardiovascular Center and the Doshisha University. Transgenic line Tg(myl7:EGFP-CAAX)ncv Tg was established by coinjecting pTol, vector-based plasmids62, containing the myl, promoter, and the enhanced green fluorescence protein (EGFP) gene fused to the CAAX motif, with Tol, transposase mRNA into wild-type (AB) embryos at one- to two-cell stage. Embryos were raised at, ., u00b0C and staged according to hours post-fertilization (hpf) and . . .
  

   

  

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