Noncovalent binding of small ubiquitin-related modifier (sumo) protease to sumo is necessary for enzymatic activities and cell growth*

HIGHLIGHTS

• who: Motomasa Ihara from the EXPERIMENTAL PROCEDURES Materials and Chemicals-pFlag-CMV2/mouse, and pFlag-CMV2/mouseSENP C A, pGEX TK/SUMO-3(GG), pT-E E SUMO-1(GG), and pT-E E SUMO-3(GG), pRS GAL-Myc-BS, pRS425, and pUC-, were provided by DrsT. Nishida (Tokyo University of Pharmacy, Tokyo, Japan), K. Tanaka (Hokkaido University, Sapporo, Japan), E. Tsuchiya (Hiroshima University, Higashi-Hiroshima, Japan), and M. Yukawa (Hiroshima University, Higashi-Hiroshima, Japan), respectively. Yeast strains , and , and plasmid p, were kindly given by Y. Kikuchi (University of Tokyo, Tokyo, Japan) (18, ). , and YCplac22 . . .
  

   

  

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