Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium

HIGHLIGHTS

• who: Alessandro Alunni and collaborators from the Whole dissected adult brains were incubated at, u00b0C for , hours in , ng/u00b5l DIG- and fluorescein-labeled mRNA probes for notch3, notch1b, jagged1a, jagged1b and jagged, (plasmids provided by Julian Lewis`s lab, London Research Institute, UK) and in DIG-labeled deltaA, deltaB and deltaD probes (provided by Bruce Appel, University of Colorado, Aurora, CO, USA)Next, cross-sections were vibratome cut and incubated with antiDIG POD (sheep, Roche, :500) or anti-Fluo POD (sheep, Roche, :500). The signal was revealed using home-made FITC and Cy3-conjugated tyramide (http . . .
  

   

  

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