Novel β-hydroxyacid dehydrogenases in escherichia coli and haemophilus influenzae *

HIGHLIGHTS

• who: Reuben K. Njau from the Methods-Ni2⫹-nitrilotriacetic acid-agarose was from QiagenPlasmid and , DNA purification reagents were from Qiagen. All PCR reagents were from PerkinElmer Life Sciences. High molecular weight genomic DNA from E. coli strain , was from Sigma., and, ␤-hydroxyisobutyrate was prepared from the corresponding methyl esters as described previously (6). Tartronate semialdehyde was prepared according to the method of Kohn and Jakoby (9). Enzyme substrates, NAD⫹, NADH, and NADP⫹ were from Sigma. pTrcHisTOPO vector was from Invitrogen. Bacterial cell culture reagents were from Life Technologies, Inc. All other chemicals, unless otherwise stated were . . .
  

   

  

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