Novel mycobacteria antigen 85 complex binding motif on fibronectin*

HIGHLIGHTS

• who: Chih-Jung Kuo from the sodium phosphate monobasic, sodium phosphate dibasic, Tris, magnesium chloride, manganese chloride, zinc chloride, copper chloride, and calcium chloride were purchased from SigmaThe Fn cell-binding domain (CBD), the Fn, kDa domain, and mouse anti-fibronectin were ordered from Millipore (Billerica, MA). Mouse anti-␣-actin antibody, mouse anti-histidine tag, mouse anti-GST antibody, and HRP-conjugated goat antimouse antibody were purchased from Invitrogen. HRP-conjugated goat anti-mouse IgG antibody and, ⬘, , ⬘-tetramethylbenzidine (TMB) peroxidase substrate were purchased from Kirkegaard and Perry Laboratories (Gaithersburg, MD). Polyclonal M. tuberculosis Ag, antibody was purchased . . .
  

   

  

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