Novel role for caspase 1 inhibitor vx765 in suppressing nlrp3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis

HIGHLIGHTS

• who: Ying Jin from the Cell culture, plasmids and transfection Human embryonic kidney , T (HEK T) cells, Jurkat T cells, mouse , macrophages, J A., macrophages, and , cells were purchased from ATCC (Manassas, VA). The cells were maintained in Dulbecco`s modified Eagle`s medium (DMEM) or RPMI , (for Jurkat cells) supplemented with, % heat-inactivated fetal bovine serum (FBS). All cell lines were verified to be mycoplasma-free. HEK T cells were transfected with Lipofectamine , reagent (Invitrogen) as described previously [12]. Plasmids expressing Flag-NLRP3, hemagglutinin (HA)-, (never in mitosis gene a-related kinase, ), Flag-ASC, Flag-procaspase . . .
  

   

  

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