Nrf1-mediated transcriptional regulation of the proteasome requires a functional tip60 complex

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  • who: Janakiram R. Vangala from the buffer, LiCl wash buffer, and TE buffer (, mM Tris (pH, ) and , mM EDTA). Elution was done with elution buffer at , °C for , h, followed by column purification (Qiagen) to obtain purified chromatin. Quantitative PCR was used to analyze the chromatin. Primers used for analysis are shown in Table, . Cell viability assays Cells were typically grown in, well plates and treated with drugs as necessary. For quantifying cell viability, the Cell-Titer Glo kit (Promega) was used. This kit measures the level of ATP, which, in turn, is proportional to the number . . .

     

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