Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after dna damage

HIGHLIGHTS

  • who: Ahmed Waraky from the concentration of, , and , ␮M, respectively, and bFGF were dissolved in, .1% bovine serum albumin (BSA) in PBS (Hyclone, Logan, UT) and used at a final concentration of , and , ng/ml, respectively. RPMI , medium, Dulbecco's modified Eagle's medium (DMEM), collagenase, and cell culture supplements, including fetal bovine serum (FBS), non-essential amino acids (NEAA), knock-out serum replacement, glutamine, puromycin, and blasticidin were purchased from Invitrogen (Paisley, UK). mTe, stem cell medium was from Stem Cell Technologies (Grenoble, France). Cell cultures Mitotically inactive feeder cells were established by treating mouse embryonic . . .

     

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