Nucleic Acids Research NAR Breakthrough Article Uncovering translation roadblocks during the development of synthetic tRNA

HIGHLIGHTS

  • who: Sweden from the Department of Structural Biology, Stanford University, Stanford, CA, USA, Program in Biophysics have published the paper: Nucleic Acids Research NAR Breakthrough Article Uncovering translation roadblocks during the development of synthetic tRNA, in the Journal: (JOURNAL)
  • how: This result was further verified when the authors used tRNAUTu1 and tRNAUTu1A for expression of human GPx1 (a natural selenoprotein with a GTT stalling motif after the amber codon).
  • SUMMARY

      The 30S PIC was then diluted to 15 nM and loaded into the SMRT_cell. Aminoacyl-tRNA synthetases (aaRSs) acylate the 3 -terminal . . .

       

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