Nucleosome-directed replication origin licensing independent of a consensus dna sequence

HIGHLIGHTS

• who: Sai Li from the Cdt Cdt, was cloned into a pET16b vector followed by replacement of the NcoI/NdeI region with insertion of a DNA segment encoding a, u00d7 Flag tag. E. coli was transformed with the pFlag-Cdt1-pET plasmid and cells were grown in LB + , u00b5g/ml ampicillin at , u00b0C with shaking until reaching an , of, .6, at which time the culture temperature was quickly reduced to , u00b0C by shaking in an ice bath. Then IPTG (, mM) was added to induce Cdt, expression for , h at , u00b0C. Cells were then harvested by low speed . . .
  

   

  

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