HIGHLIGHTS
- who: Yujian Dai from the Animal maintenance, hormone-induced superovulation, and blastocyst collection Animal experimental protocols were approved by the Animal Care and Use Committee of Nanjing Normal University (NSD-2013-30) and were performed according to guidelines from the US National Institutes of HealthAt, weeks of age, female C BL/ J mice were intraperitoneally injected with, ., IU pregnant mare serum gonadotropin (Ningbo Second Hormone Factory, China). Forty-eight hours later, mice were intraperitoneally injected with, ., IU human chorionic gonadotropin (Ningbo Second Hormone Factory) and mated with males (1:1). Blastocysts were flushed from the uterus with . . .
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