Oligomeric state of purified transient receptor potential melastatin-1 (trpm1), a protein essential for dim light vision*

HIGHLIGHTS

• who: Melina A. Agosto from the versity of Science and Technology cDNA resource center and cloned into XbaI/EcoRI sites of pFb Recombinant bacmids were made using the Bac-to-Bac procedure (Invitrogen). Bacmid DNA was prepared after a second round of colony purification and screened by PCR with , primers. Sf, cells in, cm, flasks were transfected with , ␮l of bacmid DNA and , ␮l of Cellfectin or Cellfectin II (Invitrogen) in Grace's unsupplemented medium according to the manufacturer's instructions. The , virus stock was harvested after , days. Viruses were passaged blind: , stocks were made by infecting . . .
  

   

  

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