On-line coupling of aptamer affinity solid-phase extraction and immobilized enzyme microreactor capillary electrophoresis-mass spectrometry for the sensitive targeted bottom-up analysis of protein biomarkers

HIGHLIGHTS

• who: Hiba Salim and colleagues from the (Barcelona, Spain)Water (LC-MS grade) was supplied by Fisher Scientific (Loughborough, U.K.). Particles with immobilized trypsin were provided by Promega (Madison, WI). The DNA aptamer, modified with a , spacer arm terminated by, u2032amino (M5-15-5u2032, mer, Mr=, ) and purified by HPLC, was purchased from Integrated DNA Technologies (Coralville, IA). LOABeads AffiAmino magnetic beads (MBs) of, u03bcm diameter were purchased from Lab on a Bead (Uppsala, Sweden). Electrolyte Solutions, Sheath Liquid, Protein Standards, and Blood Samples. The BGE containing , mM HAc and , mM HFor (pH, .3) was filtered . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.