One-step generation of multiple gene knock-outs in the diatom phaeodactylum tricornutum by dna-free genome editing

HIGHLIGHTS

• who: Manuel Serif from the CNRS have published the research work: One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
• what: The authors demonstrate that their inactivation, by either nucleases expressed 2 from the genome or RNP complexes, leads to 5-fluoroorotic acid (5-FOA) and 2-fluoroadenine (2-FA) resistance, a property that can be used for the direct selection of the transformants.
• how: The authors evaluated the transferability of this approach by investigating another putative . . .
  

   

  

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