One to one comparison of cell-free synthesized erythropoietin conjugates modified with linear polyglycerol and polyethylene glycol

HIGHLIGHTS

• who: Paria Pouyan from the Institutthe Brandenburg University of have published the Article: One to one comparison of cell-free synthesized erythropoietin conjugates modified with linear polyglycerol and polyethylene glycol, in the Journal: Scientific Reports Scientific Reports

SUMMARY

DNA template (60 ng/µL) was added to a reaction mixture composed of 20% (v/v) lysate, 100 µM amino_acids, salts, energy components and PolyG (10 µM, biomers, Germany). For the incorporation of the non-canonical amino_acid 2 mM p-azido-l-phenylalanine, 3 µM eAzFRS and 5 µM suppressor tRNA were added. The synthesis . . .

 



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