Open and closed conformations of a sub-80 kda chagas vaccine candidate defined by a cryoem led integrative approach

HIGHLIGHTS

• What: The authors report the first three-dimensional structure of TcPOP in open and closed conformation at a resolution of 3.0 and 2.5 Angstroms respectively determined using single-pArticle cryo-electron microscopy. The authors propose expanding this methodology for regular investigations of small proteins of sub-80kDa size, especially when recalcitrant to crystallography approaches and alternating conformations in solution, with the addition of tilted data collection, which allowed the authors to reduce preferential orientation and reveal more structural details. The authors provide experimental evidence on the transition between open and closed conformation, which will be . . .
  

   

  

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